Figure 1.

Exercise preferentially mobilizes effector lymphocytes with phenotypic and gene expression profiles associated with anti-tumor immunity. (A) Fold changes in absolute number of immune cell subsets in human whole blood from rest to during exercise, using the values presented in Table 1 (exercise/rest) (n=16). (B) Azimuth map for human PBMCs showing the 26 clusters identified by scRNAseq at rest and during exercise. (n=3) (C) Fold changes in proportion of the single cells analyzed from rest to during exercise (exercise/rest)(n=3). (D) Heat map showing differentially expressed genes (DEG) from rest to during exercise in 7 immune cell subsets (n=3). (E) Gene sets annotated to Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms that were enriched after exercise (n=3). Statistical significance: p ≤ 0.05 for DEG; and FDR ≤ 0.25 for the gene set enrichment analysis.