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. 2023 Apr 5;19(4):e1010491. doi: 10.1371/journal.ppat.1010491

Fig 3. E2-T179N increases viral replication of MAYV and CHIKV in insect cells and causes early release.

Fig 3

A-C: Replication of WT MAYV and MAYV E2-T179N in insect and human cells. Aag2 (A) and U4.4 (B) cells were infected with WT MAYV or MAYV E2-T179N at a MOI of 0.1, while MRC-5 were infected at a MOI of 0.01 (C). Replication was assessed over time by plaque assay titration. Data represents the mean of 2 independent experiments apart from the U4.4 growth curve which is representative of three independent experiments. D-E: Replication of WT CHIKV and CHIKV E2-T179N in insect and human cells. Aag2 cells were infected with WT CHIKV or CHIKV E2-T179N at a MOI of 0.1 (D), while MRC-5 were infected at a MOI of 0.01 (E). Replication was assessed over time by plaque assay. Data represents the mean of 2 independent experiments. F: One-step growth curve of WT MAYV and MAYV E2-T179N in insect cells. Aag2 cells were infected with WT MAYV and MAYV E2-T179N at an MOI of 10. Replication was assessed over time by plaque assay. Data represent the mean of 2 independent experiments. Statistical analysis: Two-Way ANOVA using Šídák’s multiple comparisons; * = p<0.05; ** = p<0.01; **** = p<0.0001. Error bars represent the standard deviation.