Figure 10.
Neuronal electrical activities were measured using the multiple-electrode array system (MEA). Mouse PCN were pretreated with 2-APB or vehicle before exposure to a H2S donor. A, spontaneous neuronal electrical spike activities are shown. Addition of H2S-induced hyperactive electrical spike activity in Phase 1, whereas pretreatment with 2-APB modestly antagonized H2S-induced suppression of electrical activity in Phases 1 and 2. B, Spontaneous electrical spike activities in PCN were quantified. Note that pretreatment with 2-APB modestly antagonized H2S-induced suppression of electrical activity in Phase 2 (Bii). N = 4. Values are presented as mean ± standard deviation. ANOVA with post hoc Tukey HSD test was performed for statistical significance. Asterisks indicate significant differences compared to vehicle group. **p < .01 and ***p < .001. Abbreviations: 2-APB, 2-aminoethoxydiphenylborate; H2S, hydrogen sulfide; PCN, primary cortical neuronal coculture.