Figure 6.

Mouse PCN were pretreated with 6 µM dantrolene before exposure to H₂S. Intracellular Ca²⁺ levels were measured, and synchronous Ca²⁺ oscillations were analyzed. A, Traces of Ca²⁺ oscillations are shown. Note that pretreatment with dantrolene had no impact on H₂S-induced SCO in Phases 2 and 3. B, A summary of SCO peak counts in H₂S exposed cortical primary neurons in Phase 2. Note that dantrolene failed to prevent on H₂S-induced suppression of peak counts. Similar results were observed for peak width and peak decay time in Phases 2 and 3 (data not shown). N = 3. Values are presented as mean ± standard deviation. ANOVA with post hoc Tukey HSD test was performed for statistical significance. Asterisks indicate significant differences compared to vehicle group. ***p < .001. Abbreviations: H₂S, hydrogen sulfide; PCN, primary cortical neuronal coculture; SCO, synchronous calcium oscillation.