Figure 8.

Ethosuximide, lamotrigine, and nimodipine failed to prevent H₂S-induced suppression of SCO. Mouse cortical primary neurons were pretreated with ethosuximide, lamotrigine, or nimodipine before exposure to H₂S. Intracellular Ca²⁺ levels were measured, and synchronous Ca²⁺ oscillations were analyzed. A, Traces of Ca²⁺ oscillations. B, A summary of analyses of SCO activity in H₂S exposed cortical primary neurons following pretreatment with ethosuximide, lamotrigine, and nimodipine. N = 3. Values are presented as mean ± standard deviation. ANOVA with post hoc Tukey HSD test was performed for statistical significance. Asterisks indicate significant differences compared to vehicle group. *p < .05, **p < .01, and ***p < .001. Abbreviations: H₂S, hydrogen sulfide; SCO, synchronous calcium oscillation.