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. 2022 Oct 10;41(4):552–559. doi: 10.1038/s41587-022-01489-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — STARSS method 1 data of (a) rigid H2B-rsEGFP2 label and (b) flexible H2B-rsEGFP2 label in U2OS cells (each curve integrated over n = 8 cells). Living cells and a few conditions of fixation are shown. The times given in the legend refer to the incubation time with PFA. This is an extended dataset of Fig. 3c of the main manuscript, with same experimental conditions.