Figure 2.

Hypoxia leads to the translocation of histone H3 from nucleus to cytoplasm in HCC cells and promotes HCC cells metastasis (A-B) Western blot analysis the expression of histone H3 in whole cell protein, cytoplasmic protein and supernatant of Huh7 and G2 cells after normoxic and hypoxic (1% O₂) culture. (C) Huh7 and G2 were stained by immunostaining after normoxia and hypoxia. Red, histone H3; blue, nuclei. Scale bar = 25 µm. (D) The viability of Huh7 and G2 cells under normoxia and hypoxia for 24h was compared by CCK-8 assay. *P<0.05, **P<0.01, N.S. no significance. (E) Transwell migration and invasion studies were performed for 24h under normoxia and hypoxia. The numbers of migratory and invasive cells were quantified in normoxia and hypoxia of Huh7 and G2 cells. Magnification = 200×, scale bar = 50 µm.