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. 2023 Apr 9;20(5):689–701. doi: 10.7150/ijms.76580

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Histones H3 promotes HCC cells proliferation and metastasis through TLR9 in vitro (A) CCK-8 assay was performed to determine the proliferation of G2 and MHCC-97H cells with various treatments. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. # represents the comparison and analysis between Lv-control+si-control and Lv-control+si-TLR9; represents the comparison and analysis between Lv-control+si-control and Lv-H3+si-control; * represents the comparison and analysis between Lv-H3+si-control and Lv-H3+si-TLR9. (B) Colony-forming assay was performed to determine the proliferation of G2 and MHCC-97H cells with various treatments. (C) G2 and MHCC-97H cells with various treatments were seeded to migrate or invade for 24h or 48h. The number of migratory and invasive cells was quantified. Magnification = 200×, scale bar = 50 µm.