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. 2023 Apr 17;13:6256. doi: 10.1038/s41598-023-33086-2

Figure 2.

Figure 2

Inhibition of SGLT2 suppresses NPs-induced premature senescence in cultured and native ECs. (a) Cell viability of PCAECs with different doses of ENA. Data are the mean ± SEM (n = 6). ###p < 0.001 versus NC (complete media). (b–c) SA-β-gal activity was determined by X gal staining in PCA and PCAECs treated with or without NPs (10 μg/mL) alone or with ENA (0.01, 0.1, 1 μM) for 24 h. Representative staining images of PCAECs showing SA-β-gal (blue stain), and PCA rings, respectively; scale bar = 100 μM. (d) Cumulative SA‐β‐gal activity in PCAECs as a percentage of control. Results are expressed as mean ± SEM (n = 3–4). **p < 0.01; ***p < 0.001 versus PC: positive control (complete media + NPs 10 μg/mL), ###p < 0.001 vs. NC (complete media).