Day	Light cycle	Dark cycle
1	Swim in 24 °C cold water for 30 min	Keep the light on
2	Deprive of water and food	Put cage on a 45° slope
3	Deprive of water	Deprive of water
4	Restrain in a tube for 3 h	Keep the light on
5	Deprive of water and food	Deprive of water
6	Wet the bedding with 200 ml water	Put cage on a 45° slope
7	Swim in 24 °C cold water for 30 min	Keep the light on
8	Put cage in the dark	Deprive of water and food
9	Swim in 24 °C cold water for 30 min	Deprive of water and food
10	Deprive of water and food	Swim in 24 °C cold water for 30 min
11	Restrain in a tube for 3 h	Wet the bedding with 200 ml water
12	Isolate from companions for 3 h	Wet the bedding with 200 ml water
13	Deprive of water and food	Put cage on a 45° slope
14	Deprive of water	Keep the light on
15	Swim in 24 °C cold water for 30 min	Deprive of food
16	Restrain in a tube for 3 h
17	Wet the bedding with 200 ml water	Wet the bedding with 200 ml water
18	Deprive of food	Wet the bedding with 200 ml water
19	Deprive of water and food	Deprive of water
20	Put cage on a 45° slope	Wet the bedding with 200 ml water
21	Restrain in a tube for 3 h