Table 4.
Histopathological findings in the four groups of animals
| Organ | Histology control | Histology 0.0025 noael | Histology 0.01 noael | Histology 0.05 noael |
|---|---|---|---|---|
| M&F | M&F | M&F | M&F | |
| Testis | Normal architecture, cellularity of Leydig, Sertoli, and germ cells | Mild architectural distortion, hemorrhagic infiltrations, and mild to a moderate decrease in cell number of all cell types | Moderate architectural distortion, hemorrhagic infiltrations, and mild to moderate reduction in the number of all cell types | Testis with extended architectural distortion, hemorrhagic infiltrations,and a significant reduction in the number of all cell lines |
| Liver | The normal architecture of sinusoidal spaces, bile canaliculi, normal cellularity,and no sign of inflammation | Mild distortion of the architecture with accompanying focal parenchymal and portal canal lymphocytic infiltration | Moderate distortion of the architecture with accompanying focal parenchymal and portal canal lymphocytic infiltration and multinucleosis of hepatic cells | Severe distortion of the architecture with accompanying parenchymal and portal canal lymphocytic infiltration with mild disruption of the terminal plate and anisonucleosis and multinucleosis of hepatic cells |
| Heart | Normal architecture and cellularity with no sign of inflammation | Mild distortion of the muscle architecture and inflammation | Moderate distortion of the muscle architecture and inflammation | Severe distortion of the muscle architecture and inflammation |
| Lung | Normal bronchial and bronchoalveolar architecture with the limited inflammatory response (presence of lymphocytes) in the peribronchial tissue | Mild distortion of the architecture and increase of the peribronchial inflammation (elements indicative of mild bronchopneumonia and bronchoalveolitis) | Moderate distortion of the architecture, intense presence of alveolar macrophages with phagocytic activity, and intense peribronchial inflammation (elements indicative of moderate bronchopneumonia and bronchoalveolitis) | Severe distortion of the architecture, the highly intense presence of alveolar macrophages with phagocytic activity, and intense peribronchial inflammation (elements indicative of moderate bronchopneumonia and bronchoalveolitis) |
| Muscle | Normal architecture of muscle fibers with no inflammatory elements | Mild derangement of muscle fibers | Moderate derangement of muscle fibers | Severe derangement of muscle fibers |
| Brain | Normal architecture, glial cellularity with no sign of inflammation | Mild distortion of the architecture and mild decrease of glial cells with inflammation | Moderate distortion of the architecture and moderate reduction of glial cells with parallel inflammation | Severe distortion of the architecture and severe reduction of glial cells with parallel inflammation |
| Kidney | Normal architecture of the cortical, medullar portion, pelvis, and calyxes with no indication of inflammation | Mild inflammatory cell infiltration and distortion of glomerular and renal tubule architecture | Mild inflammatory cell infiltrate and mild distortion of glomerular and renal tubule architecture and presence of tubular casts | Mild to moderate inflammatory cell infiltrate and mild distortion of glomerular and renal tubule architecture with single-cell tubular necrosis and presence of tubular casts |
| Stomach | Normal architecture with no distortion of the mucosal integrity and no signs of inflammation | Mild distortion of mucosal architecture and few mucosal foci of necrosis with inflammatory cell infiltrate | Moderate distortion of mucosal architecture and necrotic lesions of mucosa with inflammatory cell infiltrate | Severe distortion of mucosal architecture and necrotic lesions of mucosa with inflammatory cell infiltrate |
| Pancreas | The normal architecture of lobules and islets of Langerhans with no inflammation | Mild distortion of architecture and mild lymphocytic inflammatory response | Moderate distortion of architecture and moderate lymphocytic inflammatory response | Severe distortion of architecture and moderate lymphocytic inflammatory response |
| Spleen | Normal architecture with dense cellularity of the red and the white pulp | Mild distortion of the architecture, mild decrease of the white pulp volume and mild increase of the red pulp volume | Moderate distortion of the architecture, moderate reduction of the white pulp volume, and moderate increase of the red pulp volume | Severe distortion of the architecture, a prominent decrease of the white pulp volume, and a pronounced increase in the red pulp volume |