Skip to main content
. 2023 Mar 13;324(5):R635–R644. doi: 10.1152/ajpregu.00189.2022

Figure 2.

Figure 2.

GFRAL signaling. A: ELISA of serum GDF15 from 2.5 mg/kg DON intraperitoneally injected wild-type mice (n = 4). ****P < 0.0001 compared with saline by unpaired t test. B: immunohistochemical staining of area postrema (100 μm scale bar) from male and female GFRAL eGFP-L10 mice injected with 2.5 mg/kg DON for cfos (n = 3). Conditioned taste avoidance (CTA) assay of female GFRAL KO and WT mice with intraperitoneal administration at 2 h (C), 4 h (D), and 24 h (E; n = 5–6). ****P < 0.0001 compared with WT saline; ####P < 0.0001 compared with GFRAL KO saline by two-way ANOVA with Tukey’s multiple comparison test. Food intake (FI) test of 5 h-fasted mice conditioned to saline or 2.5 mg/kg DON at 1 h (F), 2 h (G), 4 h (H), and 24 h (I; n = 5–10). ##P < 0.01, ###P < 0.001 compared with GFRAL KO saline by two-way ANOVA with Tukey’s multiple comparison test. I: transcripts enriched in TRAP fraction of the AP in male and female GFRALeGFP-L10 mice. Differential expression was determined by CuffDiff analysis with threshold set to P < 0.05. J: expression is represented as fragments per million (FPM; n = 12). All data are represented as means ± SE. AP, area postrema; DON, deoxynivalenol; DTR, diphtheria toxin receptor; GFRAL, GDNF family receptor α-like; WT, wild type; KO, knockout; TRAP, translating ribosome affinity purification.