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. 2023 Mar 13;324(5):R635–R644. doi: 10.1152/ajpregu.00189.2022

Figure 3.

Figure 3.

GFRAL neuronal ablation. Body weight 2 wk before and 7 wk after diphtheria toxin injection in GFRAL WT and GFRAL DTR mice (n = 18–20/group; A); two-way ANOVA with Sidak’s multiple comparison test. Time x Genotype P = 0.0093. Body composition of GFRAL WT and GFRAL DTR mice 2 wk before diphtheria toxin administration (n = 18–20/group; B) and 15 wk after diphtheria toxin administration (n = 18–20/group; C); two-way ANOVA with Tukey’s multiple comparison test. Conditioned taste avoidance (CTA) assay in male GFRAL WT and GFRAL DTR mice conditioned to saline or 2.5 mg/kg DON at 4 h (D) and 24 h (E) after the addition of two-bottle choices (n = 9–11/group); two-way ANOVA with Tukey’s multiple comparison test. ***P < 0.001, ****P < 0.0001 compared with GFRAL WT saline; ####P < 0.0001 compared with GFRAL DTR saline. Food intake test after a 4- to 5-h fast in GFRAL WT and GFRAL DTR mice with either saline or 2.5 mg/kg DON injection at 2 h (F), 4 h (G), and 24 h (H) after food return. ***P < 0.001, ****P < 0.0001 compared with GFRAL WT saline and ###P < 0.001, ####P < 0.0001 compared with GFRAL DTR saline; two-way ANOVA with Tukey’s multiple comparison test. All data are represented as means ± SE. D’Agostino-Pearson test for normality was performed on B–H. DON, deoxynivalenol; DTR, diphtheria toxin receptor; GFRAL, GDNF family receptor α-like; WT, wild type.