Fig. 6.

Vδ1 T cell-mediated killing of CML targets. Freshly sorted Vδ1 γδ T cells from healthy donors were co-cultured with CML targets for 4 h, and specific lysis was determined at 5:1 (white bars) and 10:1 (gray bars) effector/ target (E:T) ratio. (A) Summary of the results from independent experiments from five healthy donors against CML cell lines including K562 (CML blast crisis), LAMA-84 and KYO-1 and against multiple myeloma U266 cell line is shown, with the mean ± SD of sample triplicates. (B) Cytotoxic reactivity data of Vδ1 γδ T cells isolated from healthy donors against primary CML cells isolated from PBMCs from patients (P001, P002 and P014) at diagnosis. Data shown are mean ± SD of independent experiments performed in duplicates. (C) Summary of inherent cytotoxicity from eight CML patients’ autologous Vδ1 γδ T cells against primary CML cells. Patient numbers are shown. Data shown are mean ± SD of independent experiments performed in duplicates. (D) Specific lysis of CML patients’ Vδ1 γδ T cells against allogeneic primary CML cells. Patient numbers are shown. Data shown are mean ± SD of independent experiments performed in duplicates