TABLE 1.

Information collected at each study time point.

Variables	Baseline	Follow-up (month after first treatment)
		1st	3rd	6th	9th	12th
Demographic characteristics	√
Unsafe sexual behaviours	√
Other sexually transmitted diseases	√
Serum TRUST titres	√	√*	√*	√*	√*	√*
Serum TPPA test	√	√*	√*	√*	√*	√*
PCR tests for SNPs related to PBPs and Tp47	√
Clinical manifestations	√	√*	√*	√*	√*	√*

TRUST, toluidine red unheated serum test; TPPA, T. pallidum particle agglutination test; PCR, polymerase chain reaction; SNP, single-nucleotide polymorphism; PBP, penicillin-binding protein. *Follow-up was terminated when the study participant responded to treatment, i.e., when the patient exhibited both loss of clinical manifestations and a ≥4-fold decrease in TRUST titre or reversion to non-reactive status in the TRUST assay.