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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: J Thromb Haemost. 2022 Dec 22;21(3):522–533. doi: 10.1016/j.jtha.2022.10.027

Figure 2. HepG2 cells are an experimental model of fibrinogen expression.

Figure 2.

(A) Media was collected from HepG2 cells cultured in the absence or presence of IL-6 for 24 hours. Proteins were separated by reducing SDS-PAGE, transferred to polyvinylidene difluoride membrane, and probed with polyclonal rabbit anti-human fibrinogen and IR-DYE 800CW goat anti-rabbit antibodies. (B) Fibrinogen in the media was quantified by densitometry; symbols indicate mean ± standard error of the mean. (C-D) Effect of IL-6 (50 ng/mL) on fibrinogen (C) protein and (D) mRNA (N=4–6, bars indicate mean + standard error of the mean).