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. 2023 Feb 14;122(7):1240–1253. doi: 10.1016/j.bpj.2023.02.014

Table 2.

Measured (a) and derived (b) parameters describing the composite anisotropy decays of NADH with lactate dehydrogenase and NADPH with isocitrate dehydrogenase, and in ternary complexes

a NADH + LDH +Lactate NADPH + IDH +Isocitrate
R(0) 0.409 [0.399, 0.417] 0.41 [0.39, 0.43] 0.40 [0.39, 0.42] 0.40 [0.37, 0.43]
τfreerot/ns 0.39 [0.38, 0.41] 0.18 [0.17, 0.21] 0.282 [0.281, 0.288] 0.27 [0.25, 0.29]
B2 0.17 [0.15, 0.18] 0.30 [0.27, 0.35] 0.22 [0.21, 0.23] 0.32 [0.26, 0.43]
τ2cone/ns 1.1 [0.9, 1.3] 1.4 [1.2, 1.9] 0.09 [0.04, 0.13] 0.5 [0.2, 0.6]
B3 0.00 [−0.11, 0.03] 0.02 [0, 0.04] 0.00 [−0.02, 0.01] 0.00 [−0.01, 0.03]
τ3cone/ns
τslowrot/ns 38 [35, 49] 45 [40, 51] 51 [48, 57] 26 [21, 29]
b NADH + LDH +Lactate NADPH + IDH +Isocitrate
Dfree/ns−1 0.43 [0.41, 0.44] 0.9 [0.8, 1.0] 0.591 [0.578, 0.592] 0.63 [0.58, 0.66]
Dtumble/ns−1 0.0044 [0.0034, 0.0048] 0.0037 [0.0033, 0.0041] 0.0032 [0.0029, 0.0035] 0.006 [0.005, 0.008]
ψboundshort 20 [19, 21] 27 [26, 30] 23.3 [22.7, 23.9] 28 [25, 34]
Dconeshort/ns−1 0.03 [0.02, 0.05] 0.04 [0.02, 0.08] 0.5 [0.3, 1.2] 0.1 [0.0, 0.7]
ψlong 0 [−15, 8] 7 [−4, 8] 0 [−7, 5] 0 [−5, 7]

Square brackets indicate 95% confidence intervals.