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. Author manuscript; available in PMC: 2023 Apr 18.
Published in final edited form as: Nat Protoc. 2022 Jul 13;17(9):2025–2053. doi: 10.1038/s41596-022-00706-6

Figure 14. ASLM imaging of a fluorescent collagen sample.

Figure 14.

a, XY maximum intensity projection. b, YZ maximum intensity projection. The excitation and detection numerical aperture were 0.8. Collagen was covalently labeled with an Alexa Fluor 568 NHS ester prior to polymerization, as described elsewhere.52 Scale bar: 10 microns.