Fig. 3.

YAP mediated the protective role of ILA in intestinal I/R injury. A-B Gene expression level (A) (n = 4–7/group) and protein expression level (B) (Four independent experiments) in intestinal tissues. C YAP downstream gene Cyr61, Ctgf expression in the intestinal tissues (n = 4–7/group). D H&E staining of the histopathological changes of intestinal tissues, immunohistochemistry staining of intestinal barrier tight junction protein (ZO-1 and Occludin), TUNEL staining of enterocyte apoptosis. E-H Quantification of pathological scores (E), relative levels of Occludin (F) and ZO-1 (G) proteins and the proportion of TUNEL⁺ cells (H) (n = 4–7/group). Results are presented as mean ± SEM. The statistical tests employed included: one-way ANOVA followed by the Tukey test for multiple comparisons in A, C and E–H. * p < 0.05, ** p < 0.01, *** p < 0 .001, **** p < 0 .0001