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. 2023 Apr 17;14:93. doi: 10.1186/s13287-023-03296-6

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effects of LIPUS stimulation on the proliferation and inflammation of C28/I2 cells. a Flow cytometry analysis for cell apoptosis of C28/I2 cells stimulated with LIPUS. b Quantification data for apoptosis rate of C28/I2 cells. c Flow cytometry analysis for cell cycle distribution of C28/I2 cells. d Quantification data for the cell cycle of C28/I2 cells. e Representative live-dead staining images of C28/I2 stimulated with LIPUS; scale bar: 200 μm. f qPCR analysis of the expression of TNF-α, IL-1β, and IL-6 in C28/I2 cells, after LPS treatment (to produce inflammation) and LIPUS stimulation. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3)