Table 1.
Characterization of the mAbs activities against RBD of SARS-CoV-2.
| ELISA Binding Activities* | Neutralizing Activities** | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Wuhan RBD | Kappa RBD | Delta RBD | Omicron BA.1 RBD | Omicron BA.1 spike | Alpha | Beta | Gamma | Kappa | Delta | Omicron BA.1 | Isotype | |
| K-RBD-mAb-1 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | － | ＋ | ＋ | ＋ | n.d. | ＋＋ | n.d. | IgG2a; k |
| K-RBD-mAb-3 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | － | － | － | － | n.d. | － | n.d. | IgG2a; k |
| K-RBD-mAb-5 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | ＋ | － | ＋ | n.d. | － | － | IgG2a; k |
| K-RBD-mAb-7 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋ | － | ＋ | ＋ | ＋ | n.d. | ＋ | n.d. | IgG2b; k |
| K-RBD-mAb-11 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋ | － | － | － | n.d. | － | － | IgG2b; k |
| K-RBD-mAb-13 | ＋＋＋ | ＋＋＋ | ＋＋ | － | － | － | － | － | n.d. | － | n.d. | IgG1; k |
| K-RBD-mAb-14 | ＋＋＋ | ＋＋＋ | ＋＋＋ | － | － | ＋ | ＋ | ＋ | n.d. | ＋ | n.d. | IgG1; k |
| K-RBD-mAb-18 | ＋＋ | ＋＋＋ | ＋＋＋ | ＋ | － | － | － | － | n.d. | － | n.d. | IgG2a; k |
| K-RBD-mAb-22 | ＋＋＋ | ＋＋＋ | ＋＋＋ | － | － | ＋ | ＋ | ＋ | n.d. | ＋ | n.d. | IgG2a; k |
| K-RBD-mAb-23 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | － | － | － | n.d. | － | － | IgG2b; k |
| K-RBD-mAb-26 | ＋＋＋ | ＋＋＋ | ＋＋＋ | － | － | ＋ | ＋ | ＋ | n.d. | ＋ | n.d. | IgG1; k |
| K-RBD-mAb-31 | ＋＋ | ＋＋＋ | － | ＋ | － | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | IgG2a; k |
| K-RBD-mAb-32 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋ | － | ＋ | ＋ | ＋ | n.d. | ＋ | n.d. | IgG2b; k |
| K-RBD-mAb-33 | ＋＋＋ | ＋＋＋ | ＋＋ | － | － | ＋ | ＋ | ＋ | n.d. | ＋ | n.d. | IgG1; k |
| K-RBD-mAb-36 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | ＋ | ＋ | － | ＋ | n.d. | － | － | IgG2a; k |
| K-RBD-mAb-40 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | － | ＋ | ＋ | ＋ | n.d. | ＋＋ | n.d. | IgG1; k |
| K-RBD-mAb-41 | n.d. | ＋＋＋ | ＋ | n.d. | n.d. | ＋ | ＋ | ＋ | n.d. | ＋＋ | n.d. | IgG1; k |
| K-RBD-mAb-42 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋＋ | IgG2a; k |
| K-RBD-mAb-43 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | － | ＋ | ＋ | ＋ | n.d. | ＋＋ | n.d. | IgG2b; k |
| K-RBD-mAb-45 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋ | － | ＋ | ＋ | ＋ | n.d. | ＋ | n.d. | IgG1; k |
| K-RBD-mAb-53 | n.d. | ＋＋＋ | ＋＋＋ | n.d. | n.d. | ＋ | － | ＋ | n.d. | － | n.d. | IgG2a; k |
| K-RBD-mAb-54 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | － | ＋ | ＋ | ＋ | n.d. | ＋＋ | n.d. | IgG2b; k |
| K-RBD-mAb-56 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | － | ＋ | ＋ | ＋ | n.d. | ＋＋ | n.d. | IgG2b; k |
| K-RBD-mAb-57 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | ＋＋ | ＋ | ＋ | ＋ | n.d. | ＋ | － | IgG1; k |
| K-RBD-mAb-60 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | ＋＋＋ | IgG2a; k |
| K-RBD-mAb-62 | n.d. | ＋＋＋ | ＋ | ＋＋＋ | ＋＋ | ＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋＋ | ＋＋＋ | IgG2a; k |
| K-RBD-mAb-63 | － | ＋ | － | － | － | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | IgG1; k |
| K-RBD-mAb-67 | ＋＋ | ＋＋＋ | － | － | － | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | IgG1; k |
| K-RBD-mAb-68 | ＋＋＋ | ＋ | － | － | － | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | IgG1; k |
| K-RBD-mAb-71 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋ | － | ＋ | ＋ | ＋ | n.d. | ＋ | n.d. | IgG2b; k |
| K-RBD-mAb-73 | ＋＋ | ＋＋＋ | ＋＋ | ＋ | － | － | － | － | n.d. | － | n.d. | IgG2a; k |
| K-RBD-mAb-75 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋＋ | IgG2a; k |
| K-RBD-mAb-78 | ＋＋＋ | ＋＋＋ | ＋ | － | － | ＋ | ＋ | ＋ | n.d. | － | n.d. | IgG1; k |
| K-RBD-mAb-79 | ＋ | ＋＋＋ | － | ＋ | － | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | IgG2a; k |
| K-RBD-mAb-80 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | ＋ | － | － | － | n.d. | － | － | IgG2a; k |
| K-RBD-mAb-83 | n.d. | ＋＋ | ＋＋ | n.d. | n.d. | ＋ | ＋ | ＋ | n.d. | ＋＋ | n.d. | IgG1; k |
| K-RBD-mAb-85 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | ＋ | － | － | － | n.d. | － | － | IgG1; k |
| K-RBD-mAb-88 | ＋＋ | ＋ | － | － | － | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | IgG1; k |
| K-RBD-mAb-89 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | － | ＋ | ＋ | ＋ | n.d. | ＋ | n.d. | IgG1; k |
| K-RBD-mAb-91 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋ | ＋＋＋ | IgG2a; k |
| K-RBD-mAb-94 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋＋ | ＋ | n.d. | n.d. | n.d. | n.d. | ＋ | － | IgG1; k |
| K-RBD-mAb-97 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | ＋＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-98 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋ | n.d. | n.d. | n.d. | n.d. | ＋ | － | IgG1; k |
| K-RBD-mAb-104 | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | － | n.d. | n.d. | n.d. | n.d. | － | n.d. | IgG1; k |
| K-RBD-mAb-106 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-111 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-113 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-114 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-115 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-118 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋ | － | n.d. | n.d. | n.d. | n.d. | － | n.d. | IgG1; k |
| K-RBD-mAb-119 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-120 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-122 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-124 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-125 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG2a; k |
| K-RBD-mAb-126 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋ | － | n.d. | n.d. | n.d. | n.d. | － | n.d. | IgG1; k |
| K-RBD-mAb-128 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | － | n.d. | n.d. | n.d. | n.d. | － | n.d. | IgG2a; k |
| K-RBD-mAb-129 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG1; k |
| K-RBD-mAb-131 | ＋＋ | ＋＋＋ | ＋ | ＋ | － | n.d. | n.d. | n.d. | n.d. | － | n.d. | IgG1; k |
| K-RBD-mAb-133 | ＋＋ | ＋＋＋ | ＋＋ | － | － | n.d. | n.d. | n.d. | n.d. | ＋＋ | n.d. | IgG1; k |
| K-RBD-mAb-137 | ＋＋＋ | ＋＋＋ | ＋＋＋ | ＋＋ | ＋ | n.d. | n.d. | n.d. | n.d. | － | － | IgG2a; k |
| NMIgG | － | － | － | － | － | － | － | － | － | － | － | |
Note: +, positive results; －, negative results; n.d., not determined; ELISA*: OD = 0.25–0.75, +, weak; OD = 0.75–1.5, ++, medium; OD > 1.5, +++, strong.**Neutralizing: +, IC 50 = 1000–100 ng/ml; ++, IC50 = 100–10 ng/ml; +++, IC50 < 10 ng/ml.