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. 2023 Mar 1;37(5-6):191–203. doi: 10.1101/gad.350320.122

Figure 5.

Figure 5

Kif1c mRNA localization is required for directed cell migration but dispensable for mRNA trafficking. (A) Schematic of the Kif1c genomic locus. The red arrowhead and stop sign in the inset denote cut site and location of premature termination codon in LOF alleles, respectively. LOF1 and LOF2 harbor 2- and 4-bp deletions in exon 11, respectively. (B) qRT-PCR on cells with endogenous deletions; mean and SEM are shown. N = 3–9 biological replicates. (C) Representative Western blot of total protein lysates. The experiment was repeated three times. (D,E) Cells were plated sparsely on fibronectin-coated plastic dishes for 18–24 h and then tracked every 3 min for at least 5 h. (D) Plot of migration persistence over time. Fifteen or more cells per genotype were used per experiment. The experiment was repeated three or more times per genotype. Error bars show SEM. (E) Individual cell tracks from a single replicate for the given genotypes. (F) qRT-PCR measurement of mRNA enrichment in protrusions in the indicated cell lines; mean and SEM are shown. All qRT-PCR data were normalized to Ppia and Ywhaz. N = 3–4 biological replicates per condition. All statistical tests are ordinary one-way ANOVA compared with parental. (***) P < 0.001, (**) P < 0.01.