Table 1. Summary of Included Studies and Result Analysis.

AFS: acellular fish skin; dHACM: dehydrated human amnio‐chorionic membrane; DPT: deep partial thickness; STSG; split thickness skin graft; FBD: fetal bovine dermis; SPT: superficial partial thickness; SSDC: silver sulfadiazine cream 1%; DFU: diabetic foot ulcer; SOC: standard of care

Studies	Year	Study type	Test performed	Results
Michael S, Winters C, Khan M. [5]	2019	Retrospective study	AFS grafts, percentage of wound surface closure	Over half the wounds healed completely
Kirsner RS, Margolis DJ, Baldursson BT, et al. [6]	2019	Randomized controlled double-blind study	Full thickness wounds created on volunteers and treated with either AFS grafts or dHACM	Wounds treated with AFS grafts healed faster.
Stone II R, Saathoff EC, Larson DA, et al. [7]	2021	Preclinical trial	DPT wounds created on pigs and treated with either AFS graft or FBD	Wounds treated with AFS grafts healed faster compared to FBD grafts
Wallner C, Holtermann J, Drysch M, et al. [8]	2022	Retrospective case control study	SPT burns treated with Suprathel, DPT burns treated with AFS graft or a STSG	Wounds treated with AFS grafts healed faster than Suprathel or STSG
Lima Júnior EM, Moraes Filho MO, Forte AJ, et al. [9]	2019	Randomized controlled trial	SPT burn wounds treated with AFS grafts or SSDC	Burn wounds healed at the same rate with AFS graft and silver sulfadiazine cream.
Lima Júnior EM, De Moraes Filho MO, Costa BA, et al. [10]	2020	Randomized controlled trial	Superficial and DPT burn wounds treated with either AFS grafts or SSDC	Burn wounds treated with AFS grafts healed faster, required fewer dressing changes and less analgesic requirements
Lima Júnior EM, de Moraes Filho MO, Costa BA, et al. [11]	2021	Randomized controlled trial	SPT burn wounds treated with AFS grafts or SSDC in an outpatient setting	Burn wounds treated with AFS grafts healed faster, were less painful and had lower treatment costs
Winters C, Kirsner RS, Margolis DJ, Lantis JC. [12]	2020	Retrospective comparative cohort study	Cost effectiveness of DFU treatment with AFS grafts compared to SOC treatment	Reduced costs when wounds are treated with AFS grafts
Lullove EJ, Liden B, Winters C, McEneaney P, Raphael A, Lantis Ii JC. [13]	2021	Randomized controlled trial	Treatment resistant DFUs treated with collagen alginate dressing with or without AFS graft	Significantly higher number of complete wound healing seen in DFUs treated with AFS grafts
Lullove EJ, Liden B, McEneaney P, et al. [14]	2022	Randomized controlled trial	DFUs treated with either collagen alginate dressing with or without AFS graft	More wound closures seen in with AFG treatment.