Table 1.
Summary of fish samples used in the present study to investigate various aspects of Cardiocephaloides longicollis metacercarial infection, distribution and encystment in the fish brain (see Materials and methods for details). Standard length and weight of fish are given as mean ± SD (min–max). ‘Mean intens.’ indicates mean intensity, ‘Mtc distrib.’ contains the number of samples used for C. longicollis metacercariae distribution per brain regions, and ‘Prev’ C. longicollis prevalence. Spaces marked with a dash indicate that no data were produced for that category.
| Group of fish | No. fish | Standard length (cm) | Weight (g) | Prev (%) | Mean intens. | Mtc. distrib. | Infection rate |
|---|---|---|---|---|---|---|---|
| Experim. infections S. aurata | |||||||
| Lethal dose | 18 | 11.76 ± 1.63 (9.50–14.50) | 13.33 ± 1.54 (10.85–15.09) | 100 | 115.8 | – | 38.59 ± 11.83 (25.0–70.0) |
| Low dosee | 33 | 9.20 ± 1.64 (5.50–13.70) | 23.81 ± 6.10 (15.24–41.2)a | 100 | 7.5 | – | 15.03 ± 13.61 (0–62) |
| High dosee,f | 36 | 13.11 ± 2.03 (10.50–18.20) | 72.68 ± 55.93 (25.8–265.1) | 100 | 31.3 | n = 14 | 20.89 ± 15.55 (4–68.67) |
| Both doses |
69 |
11.24 ± 2.70 (5.5–18.2) |
53.63 ± 49.80 (15.24 265.1)b |
100 |
19.9 |
– |
18.09 ± 14.85 (0–68.67) |
| Wild fish | |||||||
| S. aurataf | 4 | 29.38 ± 0.95 (28.0–30.0) | 755.45 ± 118.99 (691.6–933.8) | 100 | 6.5 | n = 4 | – |
| P. erythrinus | 10 | 21.75 ± 1.62 (20.0–25.0) | 270.92 ± 72.54 (188.0–423.0) | 20 | 3.0 | n = 2 | – |
|
L. mormyrusf |
10 |
21.1 ± 1.05 (20.0–23.0) |
285.68 ± 17.88 (228.0–285.78) |
70 |
5.1 |
n = 7 |
– |
| Farmed S. aurata | |||||||
| Golfo Aranci (Italy)f | 120 | 25.1 ± 1.32 (21.0–27.0)c | 329.01 ± 140.61 (115.6–582.5) | 40 | 1.3 | n = 25 | – |
| Stintino (Italy) | 30 | 24.12 ± 1.24 (22.0–7.05)d | 418.71 ± 16.41 (399.0–464.0)d | 0 | 0 | – | – |
| Marine pond (Spain) | 39 | 24.86 ± 3.1 (21.0–33.0) | 410.82 ± 61.70 (311.0–628.86) | 25.6 | 13.1 | half brain n = 10 | – |
| Levantine farm (Spain) |
211 |
21.90 ± 2.97 (15.5–36.3) |
311.54 ± 143.23 (90.79–750.4) |
0 |
0 |
– |
– |
| Histology S. aurata | 9 | 12.29 ± 4.74 (8.36–22.1) | 95.20 ± 121.00 (13.01–297.20) | 100 | – | – | – |
Based on n = 23.
Based on n = 59.
Based on n = 25. Total length is 26.18 ± 4.02 (19.2–32.7) (n = 115).
Based on n = 25.
Used for statistical analyses testing the effect of infection dose on metacercarial infection rate.
Used for statistical analyses testing the effect of brain region on No. metacercariae.