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Anti-FliCD IgG isotypes of sera from mice immunized with FliCD. Mice were immunized with FliCD three times, and serum samples were collected. The anti-FliCD IgG isotypes of the pooled serum samples from the third immunization were measured via standard ELISA. The data are presented as the mean ± SEM (n = triplicate) (*, P < 0.05; **, P < 0.01; ns, not significant).
