FIG 5.
Immunizations of mice with FliCD provide mice with significant protection against infection by C. difficile strain R20291. Mice were challenged with C. difficile R20291 spores (106/mouse) 14 days after the third immunization of the groups of mice (n = 10) with FliCD at 10 or 20 μg/mouse/immunization or with PBS in the presence of alum. (A) Kaplan-Meier survival plots (P = 0.02 between the R20291/PBS and R20291/FliCD 20 μg groups; P = 0.0628 between the R20291/PBS and R20291/FliCD 10 μg groups). (B) Mean relative weight of all surviving mice (up to the day of death). The data are presented as the mean ± SEM (*, P < 0.05). (C and D) Frequency of diarrhea.