FIG 7.

Anti-FliCD hyperimmune serum provides mice with significant protection against infection by C. difficile strain R20291. Three groups of mice (n = 10) were i.p. administered 400 μL of anti-FliCD sera (IgG titer of 10⁷), preimmune sera, or PBS 4 h before infection with C. difficile R20291 (107 spores) in the mouse model of infection. (A) Kaplan-Meier survival plots (P = 0.0079 between groups R20291/PBS and R20291/anti-FliCD; P = 0.078 between groups R20291/PBS and R20291/preimmune serum). (B) Mean relative weight of all surviving mice (up to the day of death). The data are presented as the mean ± SEM. (C and D) Frequency of diarrhea (*, P < 0.05).