FIG 4.

BTZ-043 treatment significantly reduced M. tuberculosis-induced pathology. (A) Guinea pigs (n = 6, each) were infected subcutaneously with 1 × 10³ CFU of virulent M. tuberculosis strain H37Rv. After 14 days, treatment with a daily oral dose of 300 mg/kg of BTZ-043 was started and continued for 28 days. As a control, guinea pigs were treated with either vehicle solution or 60 mg/kg of isoniazid (INH). (B) After 4 weeks, guinea pigs were euthanized and necropsied. Granulomas at the infection site, the draining axillary lymph node, and spleen were macroscopically scored. Bars represent the group medians with ranges, and asterisks represent the level of significance as calculated by nonparametric Mann-Whitney U test (*, P ≤ 0.05; **, P ≤ 0.01).