FIG 5.

BTZ-043 treatment significantly reduced M. tuberculosis-induced necrotic lesions. Six weeks after infection and after 4 weeks of treatment, BTZ-043-treated or control animals (vehicle or INH) were euthanized and dissected and organ samples were collected. Formalin-fixed, paraffin-embedded sections of the infection site granuloma (A), the axillary lymph node (B), and the spleen (C) were HE stained, whole-slide images were obtained, and absolute granuloma areas as well as area of caseous necrosis were measured. Representative microphotographs for the respective organ are shown on the left. In the middle, the quantitative analysis of the granulomatous area is shown for the three groups. Graphs on the right depict the quantitative analysis of necrotic lesions. Bars show group means, error bars indicate the standard errors of the means, and asterisks indicate the level of significance as calculated by nonparametric Mann-Whitney U test (*, P ≤ 0.05; **, P ≤ 0.01).