FIG 4.

IncM modulates Golgi distribution around the inclusion. HeLa cells were infected at a multiplicity of infection of 0.3 with C. trachomatis strain L2/434, incM::aadA, or incM::aadA harboring a plasmid encoding IncM with a C-terminal 2HA epitope tag (pIncM-2HA). Cells were fixed with methanol at 24 h postinfection and immunolabeled with anti-GM130 (cis-Golgi network; red) and anti-C. trachomatis Hsp60 (green) antibodies, stained for nuclei with DAPI (blue), and analyzed by fluorescence microscopy. (A) Representative infected cells showing Golgi distribution around the inclusion. Scale bars, 5 μm. (B) The Golgi distribution length around the inclusion was measured using Fiji (75). (C) The area of the inclusion was measured by tracing the inclusion using Fiji (75). Values represent means ± standard errors of the means, n = 3, ≥30 cells per experiment. P values were obtained using one-way ANOVA and Dunnett’s post hoc test analysis relative to cells infected by the L2/434 strain. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.