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. 2023 Mar 23;3(4):100280. doi: 10.1016/j.xgen.2023.100280

Figure 3.

Figure 3

The proliferation rate of cell lines is influenced by the pool environment, but the highest rates were observed among failed lines with BCOR LoF mutations

(A) Cell line proportion throughout dopaminergic neuron differentiation (days 11, 30, and 52). We observed a consistent outlier behavior featured by 1–2 lines that proliferate significantly faster than other lines during pooled differentiation.

(B) Failed cell lines carrying at least one BCOR LoF mutation showed, on average, a higher proliferation rate than neurons that can differentiate successfully (D52/D0, p = 0.0015, Wilcoxon rank-sum test). Boxplot whiskers are within the 1.5 IQR value. See also Figures S3A and S3B.

(C) Cell line proportion could not be replicated between pool replicates, but it remained similar between biological replicates and showed minimal differences between technical and 10X replicates, as expected (linear regression, p<0.05). The shaded area indicates 95% confidence interval on the proportion values. See also Figure S3C.