Fig. 2: Drosophila require Sestrin to adapt to a low-leucine diet.

a, b, Loss of Sestrin reduces survival during development upon leucine starvation. The bar charts show survival (%) of larvae raised for 10 days on a chemically defined diet containing 10% of the leucine in the control diet. The P values were determined using two-proportion z-test (two-sided). The bars show the percentage of surviving larvae in each genotype and the error bars represent the 95% Wald confidence interval.

c, Sestrin is required for larval growth on a low-leucine diet. Shown are age-synchronized animals of the indicated genotypes raised 9 days on either an amino-acid-replete diet or a reduced (10%)-leucine diet. Scale bar, 1 mm.

d-i, Loss of Sestrin reduces survival of adult flies upon leucine starvation. Sesn^−/− animals show reduced lifespan when fed a diet lacking leucine (0% leucine). Survival curves of age-synchronized adult male and female animals of the indicated genotypes fed the indicated diets. (c) nWT(w¹¹¹⁸)=157; nSesn^−/−=217; (d) nWT(w¹¹¹⁸)=221; nSesn^−/−=225; (e) nWT(w¹¹¹⁸)=206; nSesn^−/−=203; (f) nWT(w¹¹¹⁸)=205; nSesn^−/−=226; (g) nWT(w¹¹¹⁸)=222; nSesn^−/−=230; (h) nWT(w¹¹¹⁸)=221; nSesn^−/−=228. See statistics in Supplementary Data 1 and Methods.

(a-i), Data are representative of three independent experiments with similar results.