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Chemically defined food “Part 1” mixture
| Part 1 (AUTOCLAVE 15 minutes) | ||
|---|---|---|
| Category | Ingredient | Amount of stock per liter |
| Gelling Agent | Agar-Type II | 10 g |
| Sugar | Sucrose | 25 g |
| Metal Ions | CaCl2*6h2o | 1 mL |
| CuSO4*5h2o | 1 mL | |
| FeSO4*7h2o | 1 mL | |
| MgSO4 (anhydrous) | 1 mL | |
| MnCl2*4h2o | 1 mL | |
| ZnSO4*7h2o | 1 mL | |
| Cholesterol | Cholesterol | 15 mL |
| Amino Acids | Tyrosine | 0.93g |
| Histidine | 50 mL | |
| Isoleucine | 50 mL | |
| Methionine | 50 mL | |
| Phenylalanine | 50 mL | |
| Threonine | 50 mL | |
| Valine | 50 mL | |
| Water | Water (milliQ) | 158 mL |
