Extended Data Table 4:
Other stock solutions
| Vitamin solution | Catalog Number | g/50mL | Suspend in: |
|---|---|---|---|
| Biotin | Sigma, B4501 | 0.001 | H2O |
| Ca pantothenate | Sigma, 21210 | 0.039 | H2O |
| Nicotinic acid | Sigma, N4126 | 0.03 | H2O |
| Pyridoxine HCl | Sigma, P9755 | 0.006 | H2O |
| Riboflavin | Sigma, R4500 | 0.003 | H2O |
| Thiamine (aneurin) | Sigma, T4625 | 0.005 | H2O |
| Folic acid solution | Catalog Number | g/50mL | Suspend in: |
| Folic acid | Sigma F8758 | 0.025 | 0.004N NaOH |
| Other Nutrients solution | Catalog Number | g/50mL | Suspend in: |
| Choline chloride | MP Biomedicals, 194639 | 0.3125 | H2O |
| Inosine | Sigma, I4125 | 0.4065 | H2O |
| Myo-Inositol | Sigma, I7508 | 0.0315 | H2O |
| Uridine | Sigma, U3003 | 0.375 | H2O |
| Methyl 4-hydroxybenzoate solution | Catalog Number | g/50mL | Suspend in: |
| Methyl 4-hydroxybenzoate | Sigma, H3647 | 5 | 95% EtOH |
| Buffer | Catalog Number | 50mL stock | |
| Glacial Acetic Acid | Millipore, AX0074 | 1.5 mL | |
| KH2PO4 | JT Baker, 3246 | 1.5 g | |
| NaHCO3 | Sigma, S8875 | 0.5 g | |
| Water | Up to 50mL | ||
| Metal Ions | Catalog Number | g/50mL | Suspend in: |
| CaCl2*6h2o | Sigma, 21108 | 12.5 | H2O |
| CuSO4*5h2o | Sigma, C7631 | 0.125 | H2O |
| FeSO4*7h2o | Sigma, F7002 | 1.25 | H2O (store −20C) |
| MgSO4 (anhydrous) | Sigma, M7506 | 12.5 | H2O |
| MnCl2*4h2o | Sigma, M3634 | 0.05 | H2O |
| ZnSO4*7h2o | Sigma, Z0251 | 1.25 | H2O |
| Cholesterol solution | Catalog Number | g/50mL | Suspend in: |
| Cholesterol | Sigma, C8253 | 1 | EtOH |