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. 2022 Jun 10;33(11-12):664–682. doi: 10.1089/hum.2021.278

Figure 2.

Figure 2.

In vivo selection of an AAV2-based peptide display library using the RC and FT platforms in human primary hepatocytes in vivo. (a) Comparison of selection kinetics between the RC and FT platforms. (b–d) The performance of the most enriched variants from RC-Ad5 selection (orange) and FT-RNA selection (blue) at the level of cell entry (DNA) (b), transgene expression (RNA/cDNA) (c), and vector EXI (RNA reads normalized to DNA reads) (d). Statistics: Mann–Whitney ****p-value: <0.0001. (e, f) Individual performance of the most abundant variants from RC-Ad5 selection (orange) and FT-RNA selection (dark blue) (b, c) at the DNA (cell entry) (e) or the RNA/cDNA (expression) (f) levels. AAV2 (lower dotted line) and NP59 (upper dotted line) were included as controls. Experiments were performed in four humanized mice that had engrafted human hepatocytes from either an adult male (circles) or an infant female (triangles) donor. Statistics are shown for each variant with a higher average performance compared with NP59. Statistics: Mann–Whitney with Holm–Sidak multiple comparison adjusted p-value: ns ≥0.05. EXI, expression index.