KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken polyclonal anti-MAP2 antibody | EnCor Biotechnology Inc | Cat#7377–062921 |
| Goat anti-chicken IgY (H+L) secondary antibody, Alexa Fluor 594 | Invitrogen | A32759 |
| Phalloidin: Alexa Fluor 488 | ThermoFisher Scientific | A12379 |
| anti-FLAG HRP Antibody | Sigma | A8592 |
| Chemicals, peptides, and recombinant proteins | ||
| 2-Br-LSD hemitartrate | BetterLife | E559/BETR-001 (Lot# 08-PS-020–2A) |
| LSD | Sigma-Aldrich | L-001-1ML |
| (+)-LSD hemitartrate | NIDA Drug Supply | Cat#7315–004 |
| LSD-d3 | Sigma-Aldrich | L-002 |
| (5-HT) Serotonin Creatinine Sulfate Monohydrate | Sigma-Aldrich | H7752–500MG |
| Dopamine | Sigma | H8502–5G |
| L-(−)-norepinephrine | Cayman Chemical | Cat#16673 |
| (−) Epinephrine | Cayman Chemical | Cat#18626 |
| Pilocarpine | Cayman Chemical | Cat#14487 |
| Histamine | Cayman Chemical | Cat#33828 |
| Volinanserin (M100,907) | Cayman Chemical | Cat#15936 |
| Ketamine hydrochloride | Bimeda Canada | Cat#7087 |
| S-(−)-Raclopride (+)-tartrate | Sigma | R121 |
| (−)-Quinpirole hydrochloride | Sigma | Q102 |
| Ketaset | Zoetis | Cat#10004027 |
| Xylazine | Covetrus | Cat#061035 |
| Meloxicam | Covetrus | Cat#049756 |
| Xylene | Supelco | Cat#1330–20-7 |
| (±)-2,5-dimethoxy-4-iodoamphetamine (DOI) hydrochloride | Cayman Chemical | Cat#13885 |
| Acetronitrile (HPLC grade) | Caledon | Cat#1401–7 |
| Ammonium hydroxide (ACS grade) | Caledon | Cat#1525–1 |
| Methanol (HPLC grade) | Caledon | Cat#6701–7 |
| Poly-L-lysine | Sigma | P2636 |
| Tetracycline | Sigma | T7660 |
| Polyethyleneimine (PEI) solution | Sigma | P3143 |
| Penicillin Streptomycin Solution | VWR | Cat#45000–652 |
| Hygromycin B | Goldbio.com | H-270–1 |
| Blasticidin S HCl | GoldBio | B-800–1 |
| Zeocin | Invitrogen | R25005 |
| DMEM | VWR | Cat#45000–306 |
| FBS | VWR | Cat#97068–085 |
| Dialyzed FBS | Omega Scientific | FB-03 |
| 10xHBSS | Invitrogen | Cat#14065–056 |
| TransIT-2020 Transfection Reagent | VWR | Cat#10766–852 |
| BSA-fatty acid free | Akron | AK8909–0100 |
| 10xHBSS | ThermoFisher Scientific | H9394–1L |
| 1 M HEPES | Gibco | Cat#15630080 |
| 1 % penicillin-streptomycin | Gibco | Cat#15140122 |
| TrypLE Express | Gibco | Cat#120605–010 |
| Poly-D-lysine | Gibco | A3890401 |
| 0.5 mM Glutamax | Gibco | Cat#35050–061 |
| B-27 Plus supplement | ThermoFisher Scientific | A3582801 |
| Neurobasal Plus | ThermoFisher Scientific | A35829–01 |
| 4% paraformaldehyde | ThermoFisher Scientific | J19943-K2 |
| Triton-X | Sigma | 9036–19-5 |
| Bovine serum albumin | BioShop | Cat#1H72356 |
| Vectashield | Vector Laboratories | H-1500 |
| Critical commercial assays | ||
| Fluo-4 Direct dye | Invitrogen | F10473 |
| Coelenterazine H | Prolume | Cat#301–1 hCTZ |
| Coelenterazine 400a (Deep Blue C) | Prolume | Cat#340–1 CTZ 400a |
| Neurite Outgrowth Staining Kit | ThermoFisher Scientific | A15001 |
| FD Rapid Golgi Stain Kit protocol | FD NeuroTechnologies, Inc | PK401 |
| Geristore Syringeable Dual-Core Resin-Ionomer | DenMat | Cat#31458550 |
| Nano-Glo(R) HiBiT Extracellular Detection System, 100mL | Promega | N2421 |
| Experimental models: Cell lines | ||
| HEK293T | ATCC | CRL-3216 |
| Flp-In T-Rex 293 Cell Line | Invitrogen | R78007 |
| Experimental models: Organisms/strains | ||
| Sprague Dawley, IGS Rat | Charles River Laboratory | Cat#001CD |
| C57BL/6J | The Jackson Laboratory | Cat#000664 |
| C57BL/6J | Charles River Laboratory | Cat#027CD |
| Recombinant DNA | ||
| Aminergic GPCR Tango cDNA | Kroeze et al. 2015108 | Addgene Cat#1000000068 |
| pcDNA5/FRT/TO-Gq-Rluc8 | Olsen et al. 202038 | Addgene Cat#140982 |
| pcDNA5/FRT/TO -GoB-Rluc8 | Olsen et al. 202038 | Addgene Cat#140977 |
| pcDNA5/FRT/TO -Gs(short)-Rluc8 | Olsen et al. 202038 | Addgene Cat#140980 |
| pcDNA3.1-GFP2-γ2 | Olsen et al. 202038 | Addgene Cat#140989 |
| pcDNA3.1- GFP2-γ9 | Olsen et al. 202038 | Addgene Cat#140991 |
| pcDNA3.1- GFP2-γ1 | Olsen et al. 202038 | Addgene Cat#140989 |
| pcDNA3.1-Gβ1 | cDNA Resource Center | Cat#GNB0100000 |
| pcDNA3.1-Gβ3 | cDNA Resource Center | Cat#GNB0300000 |
| Human-β-arrestin-2 | cDNA Resource Center | Cat#ARRB200001 |
| pcDNA3.1-GFP2-human-β-arrestin-2 | This paper | N/A |
| pcDNA3.1–5-HT1A-Rluc8 | This paper | N/A |
| pcDNA3.1–5-HT1B-Rluc8 | This paper | N/A |
| pcDNA3.1–5-HT1D-Rluc8 | This paper | N/A |
| pcDNA3.1–5-HT1e-Rluc8 | This paper | N/A |
| pcDNA3.1–5-HT1F-Rluc8 | This paper | N/A |
| pcDNA3.1–5-HT2A-Rluc8 | This paper | N/A |
| pcDNA3.1–5-HT2B-Rluc8 | This paper | N/A |
| pcDNA3.1–5-HT2C (INI)-Rluc8 | This paper | N/A |
| pcDNA3.1–5-HT4-Rluc8 | This paper | N/A |
| pcDNA3.1–5-HT5a-Rluc8 | This paper | N/A |
| pcDNA3.1–5-HT6-Rluc8 | This paper | N/A |
| pcDNA3.1-human-GRK2 | This paper | N/A |
| pcDNA3.1-HiBiT-5-HT2A | This paper | N/A |
| Software and algorithms | ||
| Prism 9 | GraphPad Software | Ver. 9.0.2 |
| SPSS Statistics 26 | IBM | Release 26.0.0.0 |
| ChemDraw Professional 16.0 | PerkinElmer | N/A |
| ChemStation Software | Agilent Technologies | G2170BA |
| AnyMaze | AnyMaze | Ver. 6.35 |
| Deep Lab Cut (DLC) | Mathis Lab | Ver 2.2.1 |
| Neurolucida 360 | MBF Bioscience | Ver. 2021 |
| Neurolucida Explorer | MBF Bioscience | Ver. 2021 |
| Xen Black image analysis software | Zeiss | Ver. 2.1 |
| Custom scripts for DLC/AnyMaze analysis | Andre Telfer | https://github.com/A-Telfer/bapipe-keypoints/tree/fd24e3c7b16bd9901db95f3bbc46efc6f13268f6 |
| LabChart | ADInstruments | ver 8.0.2 |
| Matlab | Mathworks | R2019a Update 4 |