Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 20;150(6):dev201293. doi: 10.1242/dev.201293

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023. Published by The Company of Biologists Ltd

PMC Copyright notice

Fig. 5. — Cell-basement membrane interactions in Fgfr1/2 compound mutants. (A) Cell-basement membrane (BM) interactions in the submandibular gland (SMG) were analyzed for compound Fgfr1/2 compound mutants (n=24) at E15.5 by generating 3D colocalization maps. The BM was marked by laminin (Lam). K14 lineage epithelial cells expressed GFP. High-resolution confocal imaging followed by 3D rendering was used to detect colocalization between GFP of laminin and colocalization maps were generated. Control cells formed robust cell-BM contacts (yellow arrowheads). Several GFP⁺, Fgfr1^cKO/cKO; Fgfr2^cKO/cKO cells did not interact with the BM. Scale bars: 20 µm. (B) The integrity of the BM in compound Fgfr1/2 mutants was analyzed. 3D surfaces around laminin were generated from high-resolution 3D rendered confocal images. Compound Fgfr1^cKO/cKO; Fgfr2^cKO/cKO mutants showed discontinuity along the laminin surfaces (yellow arrowheads). Scale bars: 20 µm.