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HM1 and HM2 both confer membrane affinity to SUN4. Wild-type mouse SUN4 and related Myc and EGFP double-tagged fusion constructs were transiently transfected into COS-7 cells and their respective expression patterns were analyzed 24 h after transfection. Confocal images of (A) SUN4 and (B–F) Myc immunostainings are shown in combination with the EGFP fluorescence. Images are representative of at least two experimental repeats. DNA was counterstained with Hoechst 33258. Scale bars: 10 µm.
