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. 2023 Feb 17;11(6):252. doi: 10.21037/atm-22-6346

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2023 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Knockdown of circRBM33 reduced H1975-OR cells resistance to osimertinib, inhibited migration, invasion, change the cell cycle and promoted apoptosis. (A) qRT-PCR results demonstrating the knockdown efficiency of circRBM33 after H1975-OR transfection with sh-circRBM33 plasmid. (B) OD450 values of sh-circRBM33 and sh-PLVX groups were measured by CCK-8 at 0, 24, 48, 72, and 96 h using osimertinib concentration of 10 µM, respectively. (C,D) Detection of apoptosis and cycle of sh-circRBM33 and sh-PLVX cells using an attrition cytometry assay. (E,F) Migration and invasion of sh-circRBM33 and sh-PLVX were detected by transwell assay after crystal violet staining. Magnification, 400×. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; H1975-OR, H1975 osimertinib-resistant; qRT-PCR, quantitative reverse transcriptase-polymerase chain reaction; CCK-8, cell counting kit-8.