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. 2022 Aug 5;30(3-4):309–322. doi: 10.1038/s41434-022-00358-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — TX200-TR101 Tregs were cultured for up to 75 days either unstimulated or with TCR chronically stimulated with anti-CD3/CD28-coated dynabeads, in the presence or absence of high-dose IL-2 (1000 U/ml). 3 Treg donors were used with an average CAR transduction of 47 ± 3.0% (mean ± SEM) (A) Percent viability of TX200-TR101 Tregs over time, cultured without IL-2 and with or without TCR stimulation with anti-CD3/CD28-coated dynabeads. B Percent viability of TX200-TR101 Tregs over time, cultured with high-dose IL-2 without TCR stimulation. Arrows indicate IL-2 removal (starvation) and asterisk indicates reduction of IL-2 from 1000 IU/ml to 300 IU/ml. Black circles: viability of Tregs cultured with high dose of IL-2; white squares: viability of Tregs after first IL-2 removal; white triangles: viability of Tregs after second IL-2 removal. Data are presented as mean ± SEM (n = 3 Treg batches).