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. 2023 Apr 3;30(4):527–538. doi: 10.1038/s41594-023-00960-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — A, AK1 and FPGS expression in hTSC or hTSC-derived SynT populations carrying lentiviral CRISPR constructs with no sgRNAs, sgRNAs that excise the LTR10A element highlighted in Fig. 6a, or sgRNAs that excise a control region also highlighted in Fig. 6a. B, Representative FACS profiles for HLAG immunolabelling in hTSCs and hTSC-derived EVTs. The middle panel is a no-antibody control. C, Images of cells in culture (to assess morphology) or crystal violet-stained cells after an invasion assay using Matrigel-coated chambers (either home-made chambers or from a commercial provider). Images representative from six independent experiments. Scale bars are 100 µm. D, Expression of stem cell (TEAD4) and SynT (SDC1, CSH1/CSH2) in cell populations with no sgRNAs or sgRNAs that excise the LTR10A-ENG element. No significant differences were detected in any of the qRT–PCR data after Wilcoxon tests with multiple comparisons correction. Please see the data availability statement for details on source data for panels A, B and D.