Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 18;13:6277. doi: 10.1038/s41598-023-33508-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Direct interaction of tumor antigen specific T cells with antigen in tumors. (a) Analysis of CD8 T cells in tumor and peripheral lymphoid organs. PK5L1940 tumors were implanted sc. into C57BL/6 mice and tumors, tumor-draining lymph node (TDLN), non-draining lymph node (NDLN), and spleen were analyzed for SIY-specific CD8 T cells at d14. Subplots show CD62L and Ly6C expression on SIY-specific CD8 T cell populations in the lymphoid organs versus the tumor. (b) PK5L1940 tumors were implanted sc. into Nur77-GFP mice and tumor infiltrating T cells were analyzed by flow cytometry. (i) representative flow plots. The SIY-specific T cell population of infiltrating CD8 T cells was identified by pentamer staining, and CD103 + and CD103 + CD39 + subpopulations were identified and Nur77-GFP expression was determined in each cell type. Graphs show (ii) Percent Nur77-GFP + and (iii) MFI of Nur77-GFP in each group. (c) PK5L1940 cancer cells were deleted of B2M expression using CRISPR/Cas9. (i) MHCI expression in cancer cells was detected by flow cytometry compared to isotype control antibody (gray), with cells untreated (green) or pre-treated with IFNg (red). (ii) PK5L1940 or PK5L1940-B2M^−/− were co-cultured with SIY-specific 2C cells or Ova-specific OT1 cells and assessed for expression of the activation marker CD69. (iii) PK5L1940 or PK5L1940-B2M^−/− were implanted sc. into C57BL/6 mice and harvested at d14 and analyzed for infiltrating CD8 T cells and SIY-specific CD8 T cells by flow cytometry. Key: NS = not significant; * = p < 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001. Results are representative of two or more experiments.