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. 2023 Apr 18;13:6277. doi: 10.1038/s41598-023-33508-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Decreased tumor-specific memory following anti-CD40L or anti-CD4 treatment at tumor implantation. (a) PK5L1940 pancreatic adenocarcinoma cells were injected sc. into C57BL/6 mice or C57BL/6 mice treated with anti-CD40L on d0, 1, and 2, or C57BL/6 mice treated with anti-CD4 on d-1. Control mice received no treatment. (b) Mice were treated with Lm-SIY on d21 and analyzed for SIY-specific T cells in the spleen on d28 by intracellular cytokine staining for IFNg following SIY peptide stimulation. (c) Quantitative analysis of SIY-specific cells in the spleen at d28.