Fig. 2. DNMT1 mutants develop normally, but have impaired survival and reproduction.

A Images of DNMT1g2 mutant and wild-type animals. DNMT1g2 mutants complete development and are grossly indistinguishable from wild types in external anatomy despite lacking the functional DNMT1 enzyme (scale bar = 1 mm). B Mutants appear smaller in total body size (one-way ANOVA p = 0.0248; n = 4 mutant animals, 17 control animals, and 14 wild-type animals that were injected at the egg stage along with the mutants). Body size was calculated as the sum of the head, thorax, petiole, post-petiole and gaster lengths (Supplementary Fig. 3). Horizontal bars show pairwise comparisons using Tukey’s multiple comparisons test. In injected wild-type ants, CRISPR injections at the egg stage failed to produce mutations in DNMT1. Labels of individual data points correspond to animals #5-8 in Supplementary Table 1. C DNMT1g2 mutants show survival deficits relative to wild types (log-rank test: p = 0.049). Sample sizes are given in parentheses. D Eggs laid by all G0 adults were collected and sequenced, ratios of wild-type to mutant eggs compared to ratios of wild-type to G0 adults are shown. Numbers indicate sample sizes. All sequenced eggs were wild type, while only two of the six G0 adults carried wild-type alleles (two-sided Fisher’s exact test: p = 0.0012). Source data are provided as a Source Data file.