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. 2023 Apr 18;9:65. doi: 10.1038/s41531-023-00499-9

Fig. 6. Elevated steady-state levels of OMM proteins upon stress in hiPSC-derived neurons of heterozygous PRKN variant carriers.

Fig. 6

a Representative Western blot showing MFN2 and TOM70 protein levels in the hiPSC-derived neurons under basal conditions (−) and treated with 20 µM CCCP for 15 h (+). The asterisk indicates the monoubiquitinated form of MFN2. b, c Densitometric analysis of protein bands of a PD patient with homozygous PRKN mutations (PRKN++/PD+), the carriers group (PRKN+/PD−) comprising two heterozygous carriers (PRKN+/PD− 1 and PRKN+/PD− 2, respectively color coded) and the control group (representing the mean values of three control lines per experiment). MFN2 and TOM70 levels were analyzed with the indicated antibodies, and GAPDH was used as a loading control. Molecular mass markers are in kilodaltons (kDa). For both OMM proteins, levels were calculated as the ratio of MFN2 or TOM70 upon CCCP treatment over the levels of MFN2 or TOM70 under basal conditions (non-treated, NT). n = 3–4 independent experiments, respectively. The error bar represents the mean ± SEM. Significance levels were determined using one-way ANOVA and Tukey’s post hoc test to correct for multiple comparisons. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. ns not significant.