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. 2023 Apr 5;14:1178624. doi: 10.3389/fpls.2023.1178624

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Copyright © 2023 Cao, Li, Geng, Zhang, Pan, Zhang, Wang and Hao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of TaSPL14-7A haplotypes and development of molecular markers. The top panel shows a schematic diagram of the gene and 2.5 kb promoter structure of TaSPL14-7A. The ATG start codon was designated as position 1 bp. The bottom panel shows the polymorphic sites of TaSPL14-7A. A CAPS marker named CAPS-144 was developed based on the SNP (T/C) at 144 bp. Digestion of the amplified 421 bp fragment with MseI produced fragments of 238 bp and 183 bp for TaSPL14-7A-Hap3 (T), whereas this fragment could not be digested for TaSPL14-7A-Hap1/2 (C). A CAPS marker named CAPS-4111 was developed based on the SNP (G/A) at 4111 bp. Digestion of the amplified 276 bp fragment with BglI produced fragments of 220 bp and 56 bp for TaSPL14-7A-Hap2/3 (G), whereas this fragment could not be digested for TaSPL14-7A-Hap1 (A).