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Primer sequences used for the RT-PCR.
| Gene symbol | Primer sequence (5ʹ–3ʹ) |
|---|---|
| PGC-1α | Forward:CGGTGGATGAAGACGGATTGCC |
| Reverse:ATTGTAGCTGAGCTGAGTGTTGGC | |
| Sirt1 | Forward:GCTCGCCTTGCTGTGGACTTC |
| Reverse:GTGACACAGAGATGGCTGGAACTG | |
| NRF1 | Forward:AGATGCTAATGGCCCAGATG |
| Reverse:AGCTCTGCCTGGTTGTTTGT | |
| TFAM | Forward:GCTCGCCTTGCTGTGGACTTC |
| Reverse:GTGACACAGAGATGGCTGGAACTG | |
| β-actin | Forward:TGTCACCAACTGGGACGATA |
| Reverse:GGGGTGTTGAAGGTCTCAAA |
