Table 2.
Primer sequences, primer combinations and PCR cycling conditions used to amplify S. aureus AtlA gene
| Aminoacid sequence | Nucleotide sequence (5’-3’) | Reference | |
|---|---|---|---|
| 1) EATPKV | BamHI-GAAGCGACACCTAAAGTA | This study | |
| 2) THYAVS | BamHI-ACTCACTACGCTGTAAGT | This study | |
| 3) TPTPKP | BamHI-ACACCAACACCTAAGCCA | This study | |
| 4) RSTING | KpnI-ACCATTTATCGTCGAACG | This study | |
| 5) NNGVAQ | KpnI-TTGTGCGACACCATTGTT | This study | |
| 6) LAVPAA | KpnI-TGCAGCAGGTACAGCTAA | This study | |
| Combinations | |||
| Primers | Amplification condition | Amplicon size | |
| 1 + 4 |
Initial denaturation: 94 °C x 5 min; final extension:72 °C x 10 min 30 cycles 94 °C x 40 s; 50 °C x 40 s, 72 °C x 40 s. |
240 bp | |
| 1 + 5 |
Initial denaturation: 94 °C x 5 min; final extension:72 °C x 10 min 30 cycles 94 °C x 60 s; 50 °C x 60 s, 72 °C x 90 s. |
753 bp | |
| 1 + 6 |
Initial denaturation: 94 °C x 5 min; final extension:72 °C x 10 min 30 cycles 94 °C x 60 s; 58 °C x 60 s, 72 °C x 120 s. |
1050 bp | |
| 2 + 5 |
Initial denaturation: 94 °C x 5 min; final extension:72 °C x 10 min 30 cycles 94 °C x 40 s; 50 °C x 40 s, 72 °C x 40 s. |
261 bp | |
| 3 + 6 |
Initial denaturation: 94 °C x 5 min; final extension:72 °C x 10 min 30 cycles 94 °C x 60 s; 52 °C x 60 s, 72 °C x 60 s. |
492 bp | |