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. 2023 Apr 19;45(5-6):703–717. doi: 10.1007/s10529-023-03371-1

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© The Author(s), under exclusive licence to Springer Nature B.V. 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 2 — Enzymatic digestion of pET28a-His6x.CTB.ESAT-6.MPT-64, Lane M = 1.0 kb DNA marker, Lane SD = Single digestion of pET28a-His6x.CTB.ESAT-6.MPT-64 with BamHI enzyme resulted into 6.71 kb long fragment, Lane DD = Double digestion of pET28a-His6x.CTB.ESAT-6.MPT-64 plasmid with BamHI and SacI enzyme showed two bands of sizes, 5.36 kb (vector) and 1.34 kb (insert)