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. 2023 Apr 3;72:101715. doi: 10.1016/j.molmet.2023.101715

Figure 6.

Figure 6

One week of HF feeding causes a cholesterolgenic response in skeletal muscle. (A) ChIP was performed on skeletal muscle lysates. Purified DNA and primers specific to the Sp1-binding sites in the promoter region of Hmgcr were used for qPCR (n = 4–5 per group). (B) Hmgcr mRNA expression (n = 5 per group). (C) Triad-enriched membrane cholesterol (n = 6 per group). (D) Two representative images of 1-mm sections of soleus muscle per group subjected to immunofluorescent labeling of F-actin. Note prior to imaging, all samples were de-identified to ensure an objective analysis. All images were taken in the same focal plane of the section and under identical microscopic parameters. (E) Immunofluorescence F-actin intensity/area for multiple (3–5) images captured in muscle sections per muscle per mouse (n = 3). Difference between groups was analyzed using a two-way ANOVA and Tukey's multiple comparison tests. ∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0.0005, ∗∗∗∗p < 0.0001.